KMID : 0381120170390080897
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Genes and Genomics 2017 Volume.39 No. 8 p.897 ~ p.902
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Genome based quantification of Streptococcus parauberis in multiple organs of infected olive flounder (Paralichthys olivaceus)
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Kim Hyun-Su
Kim Ah-Ran Kim Sun-Mi Nguyen Thanh Luan Lim Yun-Jin Roh Heyong-Jin Kim Nam-Eun Kim Do-Hyung Choi Yung-Hyun Kim Suhk-Mann Kim Heui-Soo Ock Mee-Sun Cha Hee-Jae
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Abstract
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Although Streptococcus parauberis is the major bacterial pathogen affecting olive flounder, the translocation and dissemination of this pathogen in infected fish are not well understood. Therefore, we conducted real-time PCR and histopathologic examination to monitor the intensity of infection in multiple organs of the olive flounder after challenge with S. parauberis through subcutaneous injection. The bacterial burden in the fish kidney, when sampled at 0, 3, and 7 dpc, was 0, 6.2?¡¾?4.5?¡¿?105, and 6.7?¡¾?5.5?¡¿?106 CFU/100 mg of tissue, respectively, indicating that the infection progressed rapidly over time. Of the ten different tissues sampled, the heart and the brain were the major target organs of S. parauberis based on highest copy number as detected by our modified real-time PCR method. Histopathologic examination also showed that S. parauberis caused severe inflammation accompanied by leucocyte infiltration, connective tissue expansion, and a loss of cardiomyocytes in the brain and heart of fish sampled at dpc 7. However, the number of S. parauberis-positive fish at 3 dpc was much higher in the spleen (6/8 fish) than in the remaining organs, suggesting that the spleen is targeted in the early stages of infection relative to the heart (2/8 fish) or brain (3/8 fish). This study provides essential information for studies to find treatments for the effective elimination of S. parauberis in target organs (i.e., the brain and heart) of olive flounder.
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KEYWORD
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Streptococcosis, Streptococcus parauberis, Olive flounder, Target organ, Real-time PCR
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